BrightLine®單波段和多波段LED濾光片組 
BrightLine®單波段和多波段LED濾光片組 Semrock從今天市場(chǎng)上受歡迎的led光源的光譜開始，設(shè)計(jì)這個(gè)濾光片系列。與傳統(tǒng)的用于標(biāo)準(zhǔn)寬頻光源(如水銀或氙氣燈)的傳統(tǒng)濾光片組相比，這些濾光片可顯著改善熒光信號(hào)。 這些濾光片組被優(yōu)化，以同時(shí)傳送亮的信號(hào)和高信噪比(對(duì)比度)，可用于使用基于LED的光引擎成像各種熒光團(tuán)。 光譜與流行的LED光引擎對(duì)齊 與傳統(tǒng)光源相比，在使用led光引擎時(shí)，每個(gè)通道的信號(hào)比傳統(tǒng)光源多10倍到100倍 該系列中的常見激發(fā)濾光片簡(jiǎn)化了不同設(shè)置配置的可視化 BrightLine Single-band LED Filter Sets BrightLine單波段LED濾光片組 濾光片/主要熒光 | 激發(fā)(CWL/BW) | 發(fā)射 (CWL/BW) | 二向色邊緣 | 濾光片組零件號(hào)/ 零組零件編號(hào) | LED-DAPI-A DAPI, Alexa Fluor® 405, BFP, Pacific BlueTM | 392/23 | 447/60 | 409 nm | LED-DAPI-A-000 LED-DAPI-A-000-ZERO | LED-CFP-A CFP (ECFP), Cerulean, SYTOX Blue, TagCFP | 438/24 | 483/32 | 458 nm | LED-CFP-A-000 LED-CFP-A-000-ZERO | LED-FITC-A FITC (Fluorescein), GFP (EGFP), Cy2TM, 5-FAM | 474/27 | 525/45 | 495 nm | LED-FITC-A-000 LED-FITC-A-000-ZERO | LED-YFP-A YFP (EYFP), Venus, Fluo-3, Rhodamine 123 | 509/22 | 544/24 | 526 nm | LED-YFP-A-000 LED-YFP-A-000-ZERO | LED-TRITC-A TRITC, SpectrumOrange, dTomato, mTangerine | 554/23 | 609/54 | 573 nm | LED-TRITC-A-000 LED-TRITC-A-000-ZERO | LED-mCherry-A mCherry, TexasRed, mKate, mRFP1 | 578/21 | 641/75 | 596 nm | LED-mCherry-A-000 LED-mCherry-A-000-ZERO | LED-Cy5-A Cy5TM, Alexa Fluor® 647, APC | 635/18 | 680/42 | 652 nm | LED-Cy5-A-000 LED-Cy5-A-000-ZERO |
“平克爾”多頻帶LED濾光片組- 單頻激發(fā)器，一個(gè)三或四波段發(fā)射器，和一個(gè)多分束器 濾光片/主要熒光 | 多通帶激發(fā) | 多通帶發(fā)射 | 多邊緣二向色 | 濾光片組零件號(hào) | LED-DA/FI/TR/Cy5-A (Quad-band Full Multiband) Blue: DAPI, BFP, Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5TM, APC, Alexa Fluor® 647 | 392/23 474/27 554/23 635/18 | 432/36 515/30 595/31 730/139 | 409 nm 493 nm 573 nm 652 nm | LED-DA/FI/TR/Cy5-A-000 LED-DA/FI/TR/Cy5-A-000- ZERO |
“Sedat”多通帶LED濾光片組-單頻帶激發(fā)器和發(fā)射器，以及一個(gè)多邊分束器 濾光片組/主要熒光 | 單波段激發(fā) | 多通帶發(fā)射 | 多邊緣二向色 | 濾光片組零件號(hào) | LED-DA/FI/TX-3X-A (Triple-band Pinkel Set) | | | | LED-DA/FI/TX-3X-A-000 | Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 | Ex1: 392/23 | 432/36 | 409 nm | LED-DA/FI/TX-3X-A-000-ZERO | Green: FITC (Fluorescein), GFP (EGFP),Cy2TM | Ex2: 474/27 | 523/46 | 493 nm | | Red: Texas Red, mCherry, 5-ROX | Ex3: 575/25 | 702/196 | 596 nm | | LED-CFP/YFP/mCherry-3X-A (Triple-bandPinkelSet) | | | | LED -CFP/YFP/mCherry- | Cyan: CFP (ECFP), Cerulean, SYTOX Blue, TagCFP | Ex1: 438/24 | 475/22 | 459 nm | 3X-A-000 | Yellow: YFP (EYFP), Venus, Fluo-3, Rhodamine 123 | Ex2: 509/22 | 543/22 | 526 nm | LED -CFP/YFP/mCherry-3X-A- | Red: mCherry, TexasRed, mKate, mRFP1 | Ex3: 578/21 | 702/197 | 596 nm | 000-ZERO | LED-DA/FI/TR/Cy5-4X-A (Quad-band Pinkel Set) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5TM, APC, Alexa Fluor® 647 | Ex1: 392/23 Ex2: 474/27 Ex3: 554/23 Ex4: 635/18 | 432/36 515/30 595/31 730/139 | 409 nm 493 nm 573 nm 652 nm | LED-DA/FI/TR/Cy5-4X-A-000 LED-DA/FI/TR/Cy5-4X-A-000- ZERO |
“平克爾”多頻帶LED濾光片組- 單頻激發(fā)器，一個(gè)三或四波段發(fā)射器，和一個(gè)多分束器 濾光片組/主要熒光 | 單波段激發(fā) (CWL/BW) | 單波段發(fā)射 (CWL/BW) | 多邊緣二向色 | 濾光片組編號(hào) | LED-DA/FI/TX-3X3M-A (Triple-band Sedat Set) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Cy2TM Red: Texas Red, mCherry, 5-ROX | Ex1: 392/23 Ex2: 474/27 Ex3: 575/25 | Em1: 433/24 Em2: 525/45 Em3: 641/75 | 409 nm 493 nm 596 nm | LED-DA/FI/TX-3X3M-A-000 LED-DA/FI/TX-3X-A-000-ZERO | LED-CFP/YFP/mCherry-3X3M-A (Triple-band Sedat Set) Cyan: CFP (ECFP), Cerulean, SYTOX Blue, TagCFP Yellow: YFP (EYFP), Eosin, Fluo-3, Rhodamine 123 Red: mCherry, TexasRed, mKate, mRFP1 | Ex1: 438/24 Ex2: 509/22 Ex3: 578/21 | Em1: 482/25 Em2: 544/24 Em3: 641/75 | 459 nm 526 nm 596 nm | LED-CFP/YFP/mCherry- 3X3M-A-000 LED -CFP/YFP/mCherry-3X-A- 000-ZERO | LED-DA/FI/TR/Cy5-4X4M-A (Quad-band Sedat Set) Blue: DAPI, BFP (EBFP), Alexa Fluor® 405 Green: FITC (Fluorescein), GFP (EGFP), Alexa Fluor® 488 Orange: TRITC, DsRed, dTomato, mRFP Red: Cy5TM, APC, Alexa Fluor® 647 | Ex1: 392/23 Ex2: 474/27 Ex3: 554/23 Ex4: 635/18 | Em1: 433/24 Em2: 520/35 Em3: 600/37 Em4: 680/42 | 409 nm 493 nm 573 nm 652 nm | LED-DA/FI/TR/Cy5-4X-A-000 LED-DA/FI/TR/Cy5-4X-A-000-ZERO |
SearchLight允許熒光顯微鏡用戶和光學(xué)儀器設(shè)計(jì)者為他們的顯微鏡或系統(tǒng)預(yù)先確定z佳的熒光素、光源、探測(cè)器和光學(xué)濾鏡組合。通過消除對(duì)光譜數(shù)據(jù)源的猜測(cè)和搜索時(shí)間，探照燈用戶將能夠消除誤差并更有效的工作。用戶可以從大量的預(yù)先加載的光譜中選擇或者在這個(gè)免費(fèi)且可公開訪問的工具中上傳他們自己的光譜數(shù)據(jù)。用戶也可以安全地保存和共享數(shù)據(jù)。 共享:SearchLight的共享功能允許研究人員、工程師、公司和機(jī)構(gòu)之間建立一個(gè)*的URL鏈接，可以通過電子郵件發(fā)送給同事或合作者。 立即使用探照燈。節(jié)省時(shí)間。試一試在:http://searchlight.semrock.com 
Semrock生產(chǎn)多波段熒光濾光片，具有通帶、邊緣陡度和阻滯性能，可與z佳單波段濾光片相媲美，并且具有硬鍍膜的優(yōu)異“抗燒毀”耐久性。事實(shí)上，在每一個(gè)BrightLine濾光片組中的濾光片，包括這些多波段的濾光片，都是用同樣的耐用的硬鍍膜技術(shù)制成的。 BrightLine® Multiband Fluorescence Sets BrightLine®多波段熒光濾光片組 濾光片組/主要熒光 | 多波段激發(fā)(CWL/BW) | 多波段發(fā)射(CWL/BW) | 多邊緣二向色 | 濾光片組零件編號(hào) | DA/FI-A (Dual-Band Full Multiband) | | | | DA/FI-A-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 387/11 | 433/38 | 403 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 480/29 | 530/40 | 502 nm | | CFP/YFP-A (Dual-Band Full Multiband) | | | | CFP/YFP-A-000 | Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 | 416/25 | 464/23 | 440 nm | | Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123 | 501/18 | 547/31 | 520 nm | | GFP/DsRed-A (Dual-Band Full Multiband) | | | | GFP/DsRed-A-000 | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | 468/34 | 512/23 | 493 nm | | Red: DsRed, TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 | 553/26 | 630/91 | 574 nm | | FITC/TxRed-A (Dual-Band Full Multiband) | | | | FITC/TxRed-A-000 | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 479/38 | 524/29 | 505 nm | | Red: Texas Red®, mCherry, Alexa Fluor® 568 & 594 | 585/27 | 628/33 | 606 nm | | Cy3/Cy5-A (Dual-Band Full Multiband) | | | | Cy3/Cy5-A-000 | Yellow: Cy3™, DsRed, Alexa Fluor® 555 | 534/36 | 577/24 | 560 nm | | Red: Cy5™, SpectrumFRed™, Alexa Fluor® 647 & 660 | 635/31 | 690/50 | 659 nm | | DA/FI/TR-A (Triple-Band Full Multiband) | | | | DA/FI/TR-A-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 387/11 | 433/36 | 403 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 478/24 | 517/23 | 497 nm | | Orange: TRITC, DsRed, Cy3™, Alexa Fluor® 555 | 555/19 | 613/61 | 574 nm | | DA/FI/TX-B (Triple-Band Full Multiband) | | | | DA/FI/TX-B-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 407/14 | 457/22 | 436 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 494/20 | 530/20 | 514 nm | | Red: Texas Red®, MitoTracker Red, Alexa Fluor® 568 & 594 | 576/20 | 628/28 | 604 nm | | DA/FI/TR/Cy5-A (Quad-Band Full Multiband) | | | | DA/FI/TR/Cy5-A-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | 387/11 | 440/40 | 410 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | 485/20 | 521/21 | 504 nm | | Orange: TRITC, DsRed, Cy3™, Alexa Fluor® 555 | 559/25 | 607/34 | 582 nm | | Red: Texas Red®, MitoTracker Red, Alexa Fluor® 568 & 594 | 649/13 | 700/45 | 669 nm | |
高的透射率，阻擋度和邊緣陡度，可實(shí)現(xiàn)令人眼花繚亂的視覺和數(shù)字成像 硬的、絕緣的鍍膜為每個(gè)濾光片，包括UV激發(fā)器的“抗燒毀”性能 光譜復(fù)雜的濾光片是我們的特產(chǎn)。世界上僅有的五種彩色多波段組和大量的四、三、雙波段組可供選擇，也保證有庫存。 “平克爾”多波段濾光片組- 單頻帶激發(fā)器，一種雙-三-四或五波段發(fā)射器和一種多邊分束器 濾光片組/主要熒光團(tuán) | 多波段激發(fā)(CWL/BW) | 多波段發(fā)射(CWL/BW) | 多邊緣二向色 | 濾光片組編號(hào) | DA/FI-2X-B (Dual-Band Pinkel Set) | | | | DA/FI-2X-B-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 | Ex1: 387/11 | 433/38 | 403 nm | | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | Ex2: 485/20 | 530/40 | 502 nm | | CFP/YFP-2X-A (Dual-Band Pinkel Set) | | | | CFP/YFP-2X-A-000 | Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 | Ex1: 427/10 | 464/23 | 440 nm | | Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123 | Ex2: 504/12 | 547/31 | 520 nm | | GFP/DsRed-2X-A (Dual-Band Pinkel Set) | | | | GFP/DsRed-2X-A-000 | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | Ex1: 470/22 | 512/23 | 493 nm | | Red: DsRed, TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 | Ex2: 556/20 | 630/91 | 574 nm | | GFP/HcRed-2X-A (Dual-Band Pinkel Set) | | | | GFP/HcRed-2X-A-000 | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | Ex1: 474/23 | 527/42 | 495 nm | | Red: HcRed, Cy3.5™, Texas Red®, Alexa Fluor® 594 | Ex2: 585/29 | 645/49 | 605 nm | | FITC/TxRed-2X-B (Dual-Band Pinkel Set) | | | | FITC/TxRed-2X-B-000 | Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor® 488 | Ex1: 485/20 | 524/29 | 505 nm | | Red: Texas Red®, mCherry, Alexa Fluor® 568 & 594 | Ex2: 586/20 | 628/33 | 606 nm | | Cy3/Cy5-2X-B (Dual-Band Pinkel Set) | | | | Cy3/Cy5-2X-B-000 | Yellow: Cy3™, DsRed, Alexa Fluor® 555 | Ex1: 534/30 | 577/24 | 560 nm | | Red: Cy5™, SpectrumFRed™, Alexa Fluor® 647 & 660 | Ex2: 628/40 | 690/50 | 659 nm | | BFP/GFP/HcRed-3X-A (Triple-Band Pinkel Set) | | | | BFP/GFP/HcRed-3X-A-000 | Blue: BFP, DAPI, Hoechst, AMCA, Alexa Fluor® 350 | Ex1: 370/36 | 425/35 | 395 nm | | Green: GFP, rsGFP, FITC, Alexa Fluor® 488 | Ex2: 474/23 | 527/42 | 495 nm | | Red: HcRed, Cy3.5™, Texas Red®, Alexa Fluor® 594 | Ex3: 585/29 | 685/130 | 610 nm | | CFP/YFP/HcRed-3X-A (Triple-Band Pinkel Set) | | | | CFP/YFP/HcRed-3X-A-000 | Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 | Ex1: 427/10 | 464/23 | 444 nm | | Yellow: YFP, Calcium Green-1, Fluo-3, Rhodamine 123 | Ex2: 504/12 | 542/27 | 521 nm | | Red: HcRed, Cy3.5™, Texas Red®, Alexa Fluor® 594 | Ex3: 589/15 | 639/42 | 608 nm | | DA/FI/TR-3X-A (Triple-Band Pinkel Set) | | | | DA/FI/TR-3X-A-000 | Blue: DAPI | Ex1: 387/11 | 433/36 | 403 nm | | Green: FITC (Fluorescein), GFP (EGFP) | Ex2: 480/17 | 517/23 | 497 nm | | Orange: TRITC (Tetramethylrhodamine) | Ex3: 556/20 | 613/61 | 574 nm | | DA/FI/TX-3X-C (Triple-Band Pinkel Set) | | | | DA/FI/TX-3X-C-000 | Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor® 350 Green: FITC, GFP, rsGFP, BoDipy, Alexa Fluor® 488 | Ex1: 387/11 Ex2: 494/20 | 457/22 530/20 | 436 nm 514 nm | | Red: Texas Red®, MitoTracker Red, Alexa Fluor® 568 & 594 | Ex3: 575/25 | 628/28 | 604 nm | | DA/FI/TR/Cy5-4X-B (Quad-Band Pinkel Set) Blue: DAPI, Hoechst, AMCA, Alexa Fluor® 350 Green: FITC, GFP, rsGFP, Bodipy, AlexaFluor® 488 Orange: TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor® 647 & 660 | Ex1: 387/11 Ex2: 485/20 Ex3: 560/25 Ex4: 650/13 | 440/40 521/21 607/34 700/45 | 410 nm 504 nm 582 nm 669 nm | DA/FI/TR/Cy5-4X-B-000 | DA/FI/TR/Cy5/Cy7-5X-A (Penta-Band Pinkel Set) Blue: DAPI, Hoechst, AMCA, Alexa Fluor® 350 Green: FITC, GFP, rsGFP, Bodipy, AlexaFluor® 488 Orange: TRITC, Cy3™, Texas Red®, Alexa Fluor® 568 & 594 Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor® 647 & 660 Deep Red: Cy7™ | Ex1: 387/11 Ex2: 485/20 Ex3: 560/25 Ex4: 650/13 Ex5: 740/13 | 440/40 521/21 607/34 694/35 809/81 | 408 nm 504 nm 581 nm 667 nm 762 nm | DA/FI/TR/Cy5/Cy7 -5X-A-000 |
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